Journal: Stem Cell Reports
Article Title: Enhanced Functional Recovery from Spinal Cord Injury in Aged Mice after Stem Cell Transplantation through HGF Induction
doi: 10.1016/j.stemcr.2017.01.013
Figure Lengend Snippet: NSC TP Is as Effective in Aged Mice as It Is in Young Mice after SCI (A and B) Representative bioluminescence images and photon-count quantifications for young and aged mice after TP on days 0 (A), 7, 21, and 35 (B) (n = 10 mice/group). ∗∗ p < 0.01. (C) Representative GFP-immunostained images of sagittally sectioned spinal cords collected 5 weeks after TP. Scale bars, 500 μm (left), 50 μm (right). (D) Axial sections of the spinal cord after TP were stained for GFP, and the GFP-positive area was quantified (n = 5 mice/group). ∗ p < 0.05, ∗∗ p < 0.01. (E) Representative images stained for GFP and bromodeoxyuridine (BrdU). The experimental plan is illustrated in (F), upper panel. Arrowheads indicate GFP + BrdU + cells. Scale bar, 50 μm. (F) Quantification of BrdU + GFP + NSCs from the experiments shown in (E) (n = 4 mice/group). ∗ p < 0.05. (G) Time courses of the BMS scores of vehicle control mice (vehicle) and mice with NSC TP (n = 10 mice/group). ∗ p < 0.05, ∗∗ p < 0.01, †† p < 0.01. (H) Rotarod treadmill test performed 6 weeks after SCI (n = 10 mice/group). ∗∗ p < 0.01, †† p < 0.01. Values are means with SEM.
Article Snippet: The motor function of the hind limbs after SCI was evaluated using the locomotor rating test of the BMS ( ) and the rotarod treadmill test ( ) (Muromachi Kikai).
Techniques: Staining, Control